Composite

Part:BBa_K3790225:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-17)


GFP controlled by σS promoter csiD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


Design Notes

The following primers were used for constructing this part. 

F_A1csiD1_1035	TTTTCAGAAATCTAGATATTTTTAGATTAACTAGAGAAAGAGGAGAAATACTAGATGC	
R_A1csiD1_1907	AATATCTAGATTTCTGAAAAATGCGCACACTCCCACTCCACCTGAACC

Source

References